|Year : 2022 | Volume
| Issue : 4 | Page : 344-349
The effect of avocado seed extract (Persea Americana) on carbide induced hepatoxicity in adult Wistar rats
Nneoma V Okpala1, Joseph I Adinma2, Mark M Edet3, Damian N Ezejindu1
1 Department of Human Anatomy, Nnamdi Azikiwe University, Nnewi, Nigeria
2 Department of Obstetrics and Gynecology, Nnamdi Azikiwe University and Teaching Hospital, Nnewi, Nigeria; Centre for Health and Allied Legal and Demographical Development Research and Training (CHALADDRAT), Nnamdi Azikiwe University, Awka, Nigeria
3 Centre for Health and Allied Legal and Demographical Development Research and Training (CHALADDRAT), Nnamdi Azikiwe University, Awka, Nigeria; Department of Human Biochemistry, Nnamdi Azikiwe University, Nnewi, Nigeria
|Date of Submission||23-Jan-2022|
|Date of Decision||25-Mar-2022|
|Date of Acceptance||11-Jun-2022|
|Date of Web Publication||22-Sep-2022|
Joseph I Adinma
Department of Obstetrics and Gynecology, Nnamdi Azikiwe University and Teaching Hospital, Nnewi
Source of Support: None, Conflict of Interest: None
Background: Persea Americana is reported to have medicinal value. Calcium Carbide (CaC2), a fruit-ripening agent, has been shown to be toxic to body organs. Objective: To determine the effect of avocado seed extract (ASE) on CaC2-induced hepatotoxicity in rats. Materials and Methods: Four experimental groups, each of 5 adult wistar rats were fed respectively with normal diets (group A); 250 mg/kg ASE (group B); 100 mg/kg CaC2 (group C); combination of 100 mg/kg CaC2 and 250 mg/kg ASE – group D. Changes in the rats’ behaviours, body weights, and liver function were assessed over 21 days. Data were analyzed using SPSS. The cumulated rat weights, rat liver weights and serum liver enzyme levels for groups B, C, and D rats were compared with group A. Results: Abnormal behavior was most observed in group C rats. Rats in group A showed a significant increase in weight before and three weeks following administration of feeds while rats in groups B, C, and D showed significant decrease in weight – more marked in group C rats. Only the mean liver weights of rats in group C showed significant decrease compared to that in group A, P = 0.001. Similarly, only the cumulated liver enzyme levels of rats in group C demonstrated significant increase when compared to group A rats - indicating hepatotoxicity. Conclusion: In this study, hepatotoxicity occurred in CaC2-administered rats. However, the combination of CaC2 and ASE showed no liver toxicity indicating a mitigating effect of ASE to CaC2-induced hepatotoxicity.
Keywords: Avocado, calcium carbide, hepatotoxicity, liver, persea Americana, seed extract, toxicity, wistar rats
|How to cite this article:|
Okpala NV, Adinma JI, Edet MM, Ezejindu DN. The effect of avocado seed extract (Persea Americana) on carbide induced hepatoxicity in adult Wistar rats. Int J Med Health Dev 2022;27:344-9
|How to cite this URL:|
Okpala NV, Adinma JI, Edet MM, Ezejindu DN. The effect of avocado seed extract (Persea Americana) on carbide induced hepatoxicity in adult Wistar rats. Int J Med Health Dev [serial online] 2022 [cited 2022 Dec 9];27:344-9. Available from: https://www.ijmhdev.com/text.asp?2022/27/4/344/356625
| Introduction|| |
Leaves, peels, and seeds from fruits and other agro products, in the past have been regarded as ‘wastes,’ constituting environmental pollutants. In recent times however, they have been observed to be useful not only as nutrient, on account of their antioxidant properties but also medicinal, exhibiting immense therapeutic values in the treatment of various ailments including cancers.,,Persea Americana (PA) mill, commonly known as avocado pear, is an evergreen tree belonging to the family Lauraceae as well as the genus, persea. It was originally grown in Guatemala and Mexico but it is now grown in the tropics and sub-tropics, Nigeria inclusive. Amongst the Igbo-speaking group of southeastern Nigeria, the fruit is known as Ube Oyibo; while amongst the yorubas of southwest Nigeria it’s called “Igba or apoka.” Following the consumption of the fleshy part of avocado pear, the seed is oftentimes thrown away but on some occasion may be ground, milled or incorporated into foods for consumption as medicinal preparation – believed to be useful in the treatment of inflammations, hypertension, diabetes, and cancer.,
Calcium carbide is a group 14 alkaline earth compounds which in its pure form is colorless. It is indiscriminately used to hasten the ripening of fruits, and is believed to have toxic effects on some tissues/organs such as liver or kidney, thereby rendering it a threat to the health of human consumers.
This study has been undertaken to evaluate the effect of avocado seed extract on carbide induced hepatotoxicity in adult wistar rats.
The justification for this study is related to the fact that calcium carbide is freguently used to accelerate ripening of fruits in many agricultural and food processing settings but seldom are the users aware of its potential toxicity to the liver and other organs of the body. The findings from this study will therefore create the necessary awareness amongst the study population as to the potential toxic effect of the use of calcium carbide to accelerate fruit ripening. The study will further highlight the effect neutralizing effect of avocado seed extract on calcium-carbide induced organ toxicity, and thereby assigning a potential therapeutic property to this seed.
| Materials and Methods|| |
This study was carried out in the laboratory unit of the department of Anatomy, Nnamdi Azikiwe University, Nnewi, Anambra state southeastern Nigeria. Ethical approval was obtained from the ethics committee of faculty of basic medical sciences of the university. All chemicals and solvent materials including some equipment used in this study were obtained from the aforementioned laboratory. Thirty pieces of avocado pear bought from Nkwo market in Nnewi (June 2018) were allowed to ripe. Each was cut into halves and the seed extracted using seed extractor. The extracted seed was washed in clean tap water; sliced into pieces using manual grater and subsequently dried under the sun for 3 days. A laboratory miller was used to grind the dried seed into flour. The Test for acute toxicity from calcium carbide was carried out according to Dietrich Lorke method (1983).
Experimental animals and design
Twenty apparently healthy albino wistar rats weighing between 100 and 180g were bought from a local farm at Nnewi and moved to the site of the experiment. The rats were divided into 4 groups (each of 5 rats) and allowed to acclimatize for a period of two weeks during which they were fed with known weight of about 100g daily of normal growers mesh (a product of Premier Feed Mills Co. Limited in Sapele, Delta State, Nigeria) and water. The rats were weighed before the administration of the test substances commenced and thereafter weighed once weekly - on fridays of the week, before feeding. The administration of the avocado seed extract was done as follows: Group A - the control group received only water and feed daily for three weeks; group B - received only 250 mg/kg body weight daily of avocado seed extract for three weeks; Group C - received only 100 mg/kg body weight daily of calcium carbide for three weeks; while group D were given a combination of 100 mg/kg and 250 mg/kg body weight daily respectively of calcium carbide and avocado seed extract for three weeks. All administrations were made orally with syringes and cannula. The rats were sacrificed after 21days of administration by cervical dislocation. Following the sacrifice, blood sample of each rats collected from the heart with the use of a syringe was transferred to a clean non-anti-coagulated polystyrene tube, allowed to clot, and centrifuged at 3000 rpm for 5 min. The serum collected was then stored in a refrigerator for use in the experiment. The serum sample was also assayed biochemically for marker enzymes - aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). The isolated liver tissue of the rats were washed with normal saline, blotted with filter paper, and subjected to weighing with analytical weighing balance.
Statistical analysis was done using SPSS software version 23 (Windows IBM Cooperation). Analysis of variance (ANOVA) - followed by multiple comparism with Fisher’s LSD Post HOC test (where necessary), and student’s t-test was used in comparison of means of bio-functional parameters with P < 0.05 considered to be statistically significant.
| Results|| |
The distribution by physical and behavioural features of rats in the various groups before and following the administration of avocado seed extract and calcium carbide is shown in [Table 1]. During acclimatization period, the rats in all the groups showed healthy features (with smoothly laid hairs on their skin, pinkish eyes, normal skin colour and increase in size). Following the administration of the test substances, group A showed no clinical signs while the remaining groups had Labor breathing, staggering gait and decreased food intake which was more pronounced in rats in group C.
|Table 1: Distribution by physical and behavioural features of rats in the various groups before and following the administration of avocado seed extract and calcium carbide|
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[Table 2] shows the distribution of the mean weight of the rats in the various groups before and after the administration of test substance. There was a significant increase between the initial and final mean weight of the rats in group A while the mean weight of the rats in groups B, C, and D showed a significant decrease between the initial and the final weight, P < 0.05.
|Table 2: Distribution of the mean weight of the rats in the various groups before and after the administration of test substance|
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The distribution by experimental rat groups for their mean liver weights is shown in [Table 3]. There is no significant difference in the mean liver weights between the rats in group B (3.56 ± 0.39) and rats in group D (3.86 ± 0.04) when compared to that in group A, 3.42 ± 0.08 – (P = 0.076 apiece). However there was a significant increase in liver weight for the rats in group C (6.62 ± 0.20g) compared to the normal (group A), 3.42 ± 0.08 (P = 0.001).
|Table 3: Distribution by experimental rat groups for their mean liver weights|
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[Table 4] shows the distribution by mean levels of serum liver enzymes for the various groups - B, C, D compared to normal group A. Aspartate transaminase enzyme levels did not show any significant difference between the rats in group B and D compared to those in group A. However there was a significant increase in the levels for CaC2-administered rats. Similarly, there was slightly higher but insignificant levels of alanine transaminase enzymes for the rats in group B and D compared to those in A. This is in contradistinction to the level of enzyme for the rats in group C which showed a highly significant increase. The same pattern has also been exhibited for the levels of alkaline phosphatase enzyme which showed a significant increase only for rats in group C in contradistinction to those in group B and group D that showed no significant increase when compared to the levels for rats in group A.
|Table 4: Distribution by mean levels of serum liver enzymes for the various groups - B, C, D compared to normal group A|
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| Discussion|| |
In a comprehensive systematic review of the various components of avocado pear – peels, flesh, and seed on metabolic syndrome (a group of risk factors that include elevated blood glucose, hypertension, dislipidemia, and obesity – capable of resulting in type 2 diabetes mellitus and cardiovascular disease), Tabeshpour et al. reported that avocado had significant effect on lipid profile regulation. The study also observed that avocado pear can be taken as dietary supplement in the treatment of metabolic syndrome. Specifically, the literature has not documented studies especially in our study area as to the effect of avocado seed extract in relation to the organ toxicity of certain chemicals. Avocado seed extract has been used in this study to elicit its bio-clinical effect on adult wistar rats and also determine whether or not its intake mitigates the toxic effect of calcium carbide on the rat liver - considered to be the major metabolic site of the animal.
There was observed alterations in both the physical features and behavioural pattern of the rats following the administration of the study test substances. Labored breathing, staggering gait, and reduced food intake were the main observed features most marked, or severe, amongst the group of rats fed with calcium carbide alone; moderately observed amongst that fed with calcium carbide and PA extract; and only mildly exhibited by the rats fed with avocado seed extract alone. These observations are similar to the clinical features seen in humans with liver diseases who often times present with loss of appetite, body weakness, and weight loss over time, and suggests that the test substances, particularly calcium carbide (which exhibited the most marked manifestation of these features) may in fact have some toxic effect on the rat liver. Reduction in weight was also shown to a lower extent in rats fed with avocado seed extract alone and even those fed with calcium carbide combined with PA extract. This contrast with the observation made in the rats in group A who were given normal feeds and showed a significant weight gain post feed compared to before. The hypoglycemic effect of avocado seed extract manifested in this study has been similarly reported in previous studies., It has been shown in rats that aqueous concentration of avocado seed extract caused a significant decline in serum glucose concentration thereby exhibiting antihyperglycemic and hypoglycemic characteristics that portray its immense potentials for use in the treatment of diabetes. In a randomized clinical study of 26 healthy subjects, Sabate et al. concluded that the presence of D-mannoheptulose in avocado extract is likely to be responsible for its hypoglycemic effects through its inhibition of hexokinase activities and ultimate reduction of glycolysis.
This study demonstrated no significant difference in mean liver weight between the rats in group B and D, compared to those in group A. however, there was a significantly increased liver weight for the rats in group C – treated with calcium carbide alone. This suggests that calcium carbide may have a toxic effect on the liver, liver enlargement being a common clinical feature of liver disease. The fact that the same dose of calcium carbide combined with PA extract did not cause any significant increase in mean liver weight may suggest that pear extract may in fact have a mitigating effect on the impact of calcium carbide on rat liver – thereby probably assigning potential therapeutic properties to the Persea American seed extract. Ample evidence exist to show that in spite of the perceived therapeutic properties ascribable to PA on account of its enormous nutrient contents, avocado poising does occur in various animals – fish, cats, dogs, cattle, and rats following consumption of different components of the avocado – leaves, fruits, stem, bark and pit in variable quantities., This underscores the necessity for more comprehensive and painstaking research towards determining appropriate dosages of avocado extract compatible with safety and optimum effectiveness. Liver enzymes are important biochemical components of liver function test (LFT). Aspartate and alanine transaminase enzymes are major mirrors of derangement in function of hepatocytes, while alkaline phosphatase enzymes indicate obstruction. This study shows that the various liver enzymes exhibited no significant derangement for the rats treated with avocado extracts alone and those treated with avocado extracts in combination with calcium carbide when compared to the untreated rats. This suggests that avocado seed extract has no toxic consequences on the liver. The rats treated with calcium carbide however showed significant increase in the levels of these enzymes suggesting that the chemical may indeed have a toxic effect on the liver. The fact that the enzyme levels were not significantly raised for the rats in group D treated with a combination of calcium carbide and avocado pear indicate that avocado pear extract may, as observed with the liver have a mitigating effect on the toxicity of the calcium carbide, thereby suggesting as observed earlier that avocado pear may in fact have protective medicinal properties.
| Conclusion|| |
Avocado pear has been a time-acclaimed fruits of which it’s various components including leaf, fruits, stem, bark, and seed have been reported to be useful for their antioxidant and other therapeutic values. This study shows that the seed extract has an effect in the lowering of the weight of the rats which is believed to be on account of its antiglycemic and hypoglycemic properties. The study also demonstrates that the seed extract has no significant toxic effect on the liver evident from the insignificant deleterious effect it has in liver weights and liver enzyme levels. CaC2 on the other hand, a substance used often to effect premature ripening of fruits has been demonstrated in this study to significantly affect rat body weight and rat liver function evidenced from its effect on liver enlargement as well as derangements of liver function from significantly elevated liver enzymes. More importantly, the study has demonstrated that when administered in combination with calcium carbide, liver morphology and liver function were not significantly affected and this suggests therefore that avocado extract mitigates the deleterious effect of CaC2 on rat liver thereby assigning potential therapeutic properties to avocado seed extract. The fact that some reports have demonstrated poisoning, sometimes with lethal outcome, to some animals unequivocally suggest the need for further research into the various components of avocado to a certain appropriate dosages that will enhance their effectiveness and use on humans.
The study has been undertaken in wistar rats and the findings may not be total representative of what could occur in human subjects.
A clinical trial on the therapeutic role of avocado seed extract employing lay down process, procedures and ethical requirements is recommended.
Financial support and sponsorship
Conflicts of interest
The authors have no particular interest with respect to the publication of this work.
NVO: project administration, resources, original draft preparation; JIBDA: writing – original draft preparation, review and editing; MME: writing – original draft preparation, review and editing, data curation and visualization – where applicable; DNE: project administration, supervision.
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[Table 1], [Table 2], [Table 3], [Table 4]